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endothelin 1 et 1 receptor antagonist  (MedChemExpress)
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Ca² + <t>and</t> <t>endothelin-1</t> <t>(ET-1)</t> contribute to Piezo1-dependent activation of hypoxia-inducible factor 1-alpha (HIF-1α). a) and b) Gene set enrichment analysis (GSEA) of epidural scar tissue from mice after laminectomy. c) Ca² + influx measured by Fluo-4 AM in the blank, type I rat tail collagen-encapsulated, and collagen-encapsulated with GsMTX4 groups. d) Western blot analysis of NIH/3T3 fibroblasts treated with type I rat tail collagen and the calcium influx blocker Nimodipine (n = 3). e) Western blot analysis of NIH/3T3 fibroblasts treated with Yoda1 and Nimodipine (n = 3). f) Quantitative analysis of relative Edn1 messenger RNA (mRNA) expression in blank and collagen-encapsulated NIH/3T3 fibroblasts. g) and h) Western blot analysis of NIH/3T3 fibroblasts treated with type I rat tail collagen and the ET-1 inhibitor Bosentan (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, independent-samples t -test (two groups) or analysis of variance (multiple groups).
Endothelin 1 Et 1 Receptor Antagonist, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paraburkholderia phytofirmans psjn onion roots gcf 000020125 1  (ATCC)
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ATCC paraburkholderia phytofirmans psjn onion roots gcf 000020125 1
Ca² + <t>and</t> <t>endothelin-1</t> <t>(ET-1)</t> contribute to Piezo1-dependent activation of hypoxia-inducible factor 1-alpha (HIF-1α). a) and b) Gene set enrichment analysis (GSEA) of epidural scar tissue from mice after laminectomy. c) Ca² + influx measured by Fluo-4 AM in the blank, type I rat tail collagen-encapsulated, and collagen-encapsulated with GsMTX4 groups. d) Western blot analysis of NIH/3T3 fibroblasts treated with type I rat tail collagen and the calcium influx blocker Nimodipine (n = 3). e) Western blot analysis of NIH/3T3 fibroblasts treated with Yoda1 and Nimodipine (n = 3). f) Quantitative analysis of relative Edn1 messenger RNA (mRNA) expression in blank and collagen-encapsulated NIH/3T3 fibroblasts. g) and h) Western blot analysis of NIH/3T3 fibroblasts treated with type I rat tail collagen and the ET-1 inhibitor Bosentan (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, independent-samples t -test (two groups) or analysis of variance (multiple groups).
Paraburkholderia Phytofirmans Psjn Onion Roots Gcf 000020125 1, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s oneidensis mr 1  (ATCC)
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Ca² + <t>and</t> <t>endothelin-1</t> <t>(ET-1)</t> contribute to Piezo1-dependent activation of hypoxia-inducible factor 1-alpha (HIF-1α). a) and b) Gene set enrichment analysis (GSEA) of epidural scar tissue from mice after laminectomy. c) Ca² + influx measured by Fluo-4 AM in the blank, type I rat tail collagen-encapsulated, and collagen-encapsulated with GsMTX4 groups. d) Western blot analysis of NIH/3T3 fibroblasts treated with type I rat tail collagen and the calcium influx blocker Nimodipine (n = 3). e) Western blot analysis of NIH/3T3 fibroblasts treated with Yoda1 and Nimodipine (n = 3). f) Quantitative analysis of relative Edn1 messenger RNA (mRNA) expression in blank and collagen-encapsulated NIH/3T3 fibroblasts. g) and h) Western blot analysis of NIH/3T3 fibroblasts treated with type I rat tail collagen and the ET-1 inhibitor Bosentan (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, independent-samples t -test (two groups) or analysis of variance (multiple groups).
S Oneidensis Mr 1, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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shewanella oneidensis mr 1  (ATCC)
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ATCC shewanella oneidensis mr 1
Ca² + <t>and</t> <t>endothelin-1</t> <t>(ET-1)</t> contribute to Piezo1-dependent activation of hypoxia-inducible factor 1-alpha (HIF-1α). a) and b) Gene set enrichment analysis (GSEA) of epidural scar tissue from mice after laminectomy. c) Ca² + influx measured by Fluo-4 AM in the blank, type I rat tail collagen-encapsulated, and collagen-encapsulated with GsMTX4 groups. d) Western blot analysis of NIH/3T3 fibroblasts treated with type I rat tail collagen and the calcium influx blocker Nimodipine (n = 3). e) Western blot analysis of NIH/3T3 fibroblasts treated with Yoda1 and Nimodipine (n = 3). f) Quantitative analysis of relative Edn1 messenger RNA (mRNA) expression in blank and collagen-encapsulated NIH/3T3 fibroblasts. g) and h) Western blot analysis of NIH/3T3 fibroblasts treated with type I rat tail collagen and the ET-1 inhibitor Bosentan (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, independent-samples t -test (two groups) or analysis of variance (multiple groups).
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nk 1  (ATCC)
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ATCC nk 1
Growth and PHB degradation of marine bacteria. (A) Growth curves of Bacillus sp. NRRL B-14911 (dark green), C. testosteroni (light green), Marinobacter <t>sp.</t> <t>NK-1</t> (light brown), and Microbulbifer sp. SOL66 (dark brown) cultured in Marine Broth at 30 °C. (B) Degradation of PHB by the marine bacteria species was determined by growing bacterial colonies on Marine Broth-agar plates containing PHB powder and measuring the radii of the clear zones of depolymerized PHB formed around the colonies after 7 days ( n = 8). **** P ≤ 0.0001 by one-way ANOVA statistical analysis.
Nk 1, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasma endothelin 1 et 1 levels  (Thermo Fisher)
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Growth and PHB degradation of marine bacteria. (A) Growth curves of Bacillus sp. NRRL B-14911 (dark green), C. testosteroni (light green), Marinobacter <t>sp.</t> <t>NK-1</t> (light brown), and Microbulbifer sp. SOL66 (dark brown) cultured in Marine Broth at 30 °C. (B) Degradation of PHB by the marine bacteria species was determined by growing bacterial colonies on Marine Broth-agar plates containing PHB powder and measuring the radii of the clear zones of depolymerized PHB formed around the colonies after 7 days ( n = 8). **** P ≤ 0.0001 by one-way ANOVA statistical analysis.
Plasma Endothelin 1 Et 1 Levels, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gca 030161115 1  (ATCC)
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ATCC gca 030161115 1
Growth and PHB degradation of marine bacteria. (A) Growth curves of Bacillus sp. NRRL B-14911 (dark green), C. testosteroni (light green), Marinobacter <t>sp.</t> <t>NK-1</t> (light brown), and Microbulbifer sp. SOL66 (dark brown) cultured in Marine Broth at 30 °C. (B) Degradation of PHB by the marine bacteria species was determined by growing bacterial colonies on Marine Broth-agar plates containing PHB powder and measuring the radii of the clear zones of depolymerized PHB formed around the colonies after 7 days ( n = 8). **** P ≤ 0.0001 by one-way ANOVA statistical analysis.
Gca 030161115 1, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gca 000420245 1  (ATCC)
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ATCC gca 000420245 1
Growth and PHB degradation of marine bacteria. (A) Growth curves of Bacillus sp. NRRL B-14911 (dark green), C. testosteroni (light green), Marinobacter <t>sp.</t> <t>NK-1</t> (light brown), and Microbulbifer sp. SOL66 (dark brown) cultured in Marine Broth at 30 °C. (B) Degradation of PHB by the marine bacteria species was determined by growing bacterial colonies on Marine Broth-agar plates containing PHB powder and measuring the radii of the clear zones of depolymerized PHB formed around the colonies after 7 days ( n = 8). **** P ≤ 0.0001 by one-way ANOVA statistical analysis.
Gca 000420245 1, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Ca² + and endothelin-1 (ET-1) contribute to Piezo1-dependent activation of hypoxia-inducible factor 1-alpha (HIF-1α). a) and b) Gene set enrichment analysis (GSEA) of epidural scar tissue from mice after laminectomy. c) Ca² + influx measured by Fluo-4 AM in the blank, type I rat tail collagen-encapsulated, and collagen-encapsulated with GsMTX4 groups. d) Western blot analysis of NIH/3T3 fibroblasts treated with type I rat tail collagen and the calcium influx blocker Nimodipine (n = 3). e) Western blot analysis of NIH/3T3 fibroblasts treated with Yoda1 and Nimodipine (n = 3). f) Quantitative analysis of relative Edn1 messenger RNA (mRNA) expression in blank and collagen-encapsulated NIH/3T3 fibroblasts. g) and h) Western blot analysis of NIH/3T3 fibroblasts treated with type I rat tail collagen and the ET-1 inhibitor Bosentan (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, independent-samples t -test (two groups) or analysis of variance (multiple groups).

Journal: Bone & Joint Research

Article Title: Piezo1 drives fibroblast activation in epidural fibrotic remodelling via the ET-1/HIF-1α pathway

doi: 10.1302/2046-3758.158.BJR-2025-0662.R1

Figure Lengend Snippet: Ca² + and endothelin-1 (ET-1) contribute to Piezo1-dependent activation of hypoxia-inducible factor 1-alpha (HIF-1α). a) and b) Gene set enrichment analysis (GSEA) of epidural scar tissue from mice after laminectomy. c) Ca² + influx measured by Fluo-4 AM in the blank, type I rat tail collagen-encapsulated, and collagen-encapsulated with GsMTX4 groups. d) Western blot analysis of NIH/3T3 fibroblasts treated with type I rat tail collagen and the calcium influx blocker Nimodipine (n = 3). e) Western blot analysis of NIH/3T3 fibroblasts treated with Yoda1 and Nimodipine (n = 3). f) Quantitative analysis of relative Edn1 messenger RNA (mRNA) expression in blank and collagen-encapsulated NIH/3T3 fibroblasts. g) and h) Western blot analysis of NIH/3T3 fibroblasts treated with type I rat tail collagen and the ET-1 inhibitor Bosentan (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, independent-samples t -test (two groups) or analysis of variance (multiple groups).

Article Snippet: Alternatively, the following drugs were used to stimulate NIH/3T3 cells: Yoda1 (25 μM; HY-P1410), a chemical activator of Piezo1; BAY 87-2243 (1 μM; HY-15836), an inhibitor of hypoxia-inducible factor 1-alpha (HIF-1α); SB-431542 (10 μM; HY-10431), an inhibitor of TGF-β1; SIS 3 (10 μM; HY-13013), an inhibitor of Smad3; GsMTX4 (2.5 μM; HY-P1410); Nimodipine (60 μM; HY-B0265), a calcium channel blocker; and Bosentan (1 μM; HY-A0013, all MedChemExpress), an endothelin-1 (ET-1) receptor antagonist.

Techniques: Activation Assay, Western Blot, Expressing

Growth and PHB degradation of marine bacteria. (A) Growth curves of Bacillus sp. NRRL B-14911 (dark green), C. testosteroni (light green), Marinobacter sp. NK-1 (light brown), and Microbulbifer sp. SOL66 (dark brown) cultured in Marine Broth at 30 °C. (B) Degradation of PHB by the marine bacteria species was determined by growing bacterial colonies on Marine Broth-agar plates containing PHB powder and measuring the radii of the clear zones of depolymerized PHB formed around the colonies after 7 days ( n = 8). **** P ≤ 0.0001 by one-way ANOVA statistical analysis.

Journal: ACS Applied Polymer Materials

Article Title: 3D-Bioprinted Marine Bacteria for the Degradation of Polyhydroxybutyrate Bioplastics

doi: 10.1021/acsapm.5c03370

Figure Lengend Snippet: Growth and PHB degradation of marine bacteria. (A) Growth curves of Bacillus sp. NRRL B-14911 (dark green), C. testosteroni (light green), Marinobacter sp. NK-1 (light brown), and Microbulbifer sp. SOL66 (dark brown) cultured in Marine Broth at 30 °C. (B) Degradation of PHB by the marine bacteria species was determined by growing bacterial colonies on Marine Broth-agar plates containing PHB powder and measuring the radii of the clear zones of depolymerized PHB formed around the colonies after 7 days ( n = 8). **** P ≤ 0.0001 by one-way ANOVA statistical analysis.

Article Snippet: The bacteria strains used in this study are Bacillus sp. NRRL B-14911 , (Agricultural Research Service Culture Collection, United States Department of Agriculture), Comamonas testosteroni (ATCC 11996), Marinobacter sp. NK-1 (ATCC 700491), Microbulbifer sp. SOL66 (ATCC 70072), and Escherichia coli BL21 (ATCC BAA-1025).

Techniques: Bacteria, Cell Culture